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mouse ATP6V1H shRNA silencing Adenovirus

Name 
Ad-m-ATP6V1H-shRNA
Cat No 
shADV-253356
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Atp6v1h.

shADV-253356
Ad-m-ATP6V1H-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Atp6v1h (ATPase, H+ transporting, lysosomal V1 subunit H). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
0710001F19Rik; AU022349; CGI-11; SFD; SFDalpha; SFDbeta; VMA13
Description (Vector)
Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system (By similarity). Involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes.
Gene ID
108664
Gene Name (Vector)
ATPase, H+ transporting, lysosomal V1 subunit H
Gene Symbol
Atp6v1h
ORF Size (aa)
1452
ORF Size (bp)
1452 bp
RefSeq ID
NM_133826
RefSeq Synonyms
NM_133826, NM_001310442, BC009154,
Species
mouse
UniGene ID
Mm.27082

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.