mouse ATP5H shRNA silencing Adenovirus

Name
Ad-m-ATP5H-shRNA

Cat No
shADV-253305

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Atp5h.

shADV-253305

Ad-m-ATP5H-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Atp5h (ATP synthase, H+ transporting, mitochondrial F0 complex, subunit D). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

0610009D10Rik

Description (Vector)

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.

Gene ID

71679

Gene Name (Vector)

ATP synthase, H+ transporting, mitochondrial F0 complex, subunit D

Gene Symbol

Atp5h

ORF Size (aa)

486

RefSeq ID

NM_027862

RefSeq Synonyms

NM_027862, BC081431, BC016547,

Species

mouse

UniGene ID

Mm.371641

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.