mouse ABCA4 shRNA silencing Adenovirus

Name 
Ad-m-ABCA4-shRNA
Cat No 
shADV-251762
Availability
7-8 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Abca4.

shADV-251762
Ad-m-ABCA4-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Abca4 (ATP-binding cassette, sub-family A (ABC1), member 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
Abc10; Abcr; AW050280; D430003I15Rik; RmP
Description (Vector)
In the visual cycle, acts as an inward-directed retinoid flipase, retinoid substrates imported by ABCA4 from the extracellular or intradiscal (rod) membrane surfaces to the cytoplasmic membrane surface are all-trans-retinaldehyde (ATR) and N-retinyl-phosphatidyl-ethanolamine (NR-PE). Once transported to the cytoplasmic surface, ATR is reduced to vitamin A by trans-retinol dehydrogenase (tRDH) and then transferred to the retinal pigment epithelium (RPE) where it is converted to 11-cis-retinal. May play a role in photoresponse, removing ATR/NR-PE from the extracellular photoreceptor surfaces during bleach recovery.
Gene ID
11304
Gene Name (Vector)
ATP-binding cassette, sub-family A (ABC1), member 4
Gene Symbol
Abca4
ORF Size (aa)
6933
ORF Size (bp)
6933 bp
RefSeq ID
NM_007378
RefSeq Synonyms
NM_007378, BC057853,
Species
mouse
UniGene ID
Mm.3918

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.