human DGKE shRNA silencing Adenovirus

Name 
Ad-h-DGKE-shRNA
Cat No 
shADV-230456
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human DGKE.

shADV-230456
Ad-h-DGKE-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human DGKE (diacylglycerol kinase epsilon). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
AHUS7; DAGK5; DAGK6; DGK; NPHS7
Description (Vector)
Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis.
Gene ID
8526
Gene Name (Vector)
diacylglycerol kinase epsilon
Gene Symbol
DGKE
HGNC ID
HGNC:2852
ORF Size (aa)
1704
ORF Size (bp)
1704 bp
RefSeq ID
NM_003647
RefSeq Synonyms
NM_003647, BC130631, BC130629,
Species
human
UniGene ID
Hs.239514

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.