human DGKE shRNA silencing AAV

Name 
AAV-h-DGKE-shRNA
Cat No 
shAAV-230456
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human DGKE.

shAAV-230456
AAV-h-DGKE-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human DGKE (diacylglycerol kinase epsilon). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
AHUS7; DAGK5; DAGK6; DGK; NPHS7
Description (Vector)
Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis.
Gene ID
8526
Gene Name (Vector)
diacylglycerol kinase epsilon
Gene Symbol
DGKE
HGNC ID
HGNC:2852
ORF Size (aa)
1704
ORF Size (bp)
1704 bp
RefSeq ID
NM_003647
RefSeq Synonyms
NM_003647, BC130631, BC130629,
Species
human
UniGene ID
Hs.239514

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.