human WWP1 shRNA silencing Adenovirus

Name 
Ad-h-WWP1-shRNA
Cat No 
shADV-227858
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human WWP1.

shADV-227858
Ad-h-WWP1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human WWP1 (WW domain containing E3 ubiquitin protein ligase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
AIP5; atrophin-1 interacting protein 5; Atrophin-1-interacting protein 5; HECT-type E3 ubiquitin transferase WWP1; hSDRP1; NEDD4-like E3 ubiquitin-protein ligase WWP1; Nedd-4-like ubiquitin-protein ligase; TGIF-interacting ubiquitin ligase 1; Tiul1; WW domain-containing protein 1
Description (eCommerce)
WW domain-containing protein 1 (WWP1) functions as an E3 ubiquitin-protein ligase. WWP1 ubiquitinates ERBB4 isoforms JM-A CYT-1 and JM-B CYT-1, KLF2, KLF5 and TP63 and promotes their proteasomal degradation. WWP1 also ubiquitinates and promotes degradation of TGFBR1; TGFBR1 ubiquitination is enhanced by SMAD7, and WWP1 also ubiquitinates SMAD7 as well as SMAD6. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9H0M0].
Description (Vector)
WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. The encoded protein belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. Alternative splicing of this gene generates at least 6 transcript variants; however, the full length nature of these transcripts has not been defined.
Gene ID
11059
Gene Name (eCommerce)
WW domain containing E3 ubiquitin protein ligase 1
Gene Name (Vector)
WW domain containing E3 ubiquitin protein ligase 1
Gene Symbol
WWP1
HGNC ID
HGNC:17004
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2769
ORF Size (bp)
2769 bp
Protein Name (eCommerce)
NEDD4-like E3 ubiquitin-protein ligase WWP1
RefSeq ID
NM_007013
RefSeq Synonyms
NM_007013, BC036065,
Research Areas
Antigen Processing and Presentation,Host-Virus Interactions,Immunology,Neurobiology,Neurodevelopment,Signal Transduction,TGF-beta Signaling,Ubiquitination
Research Areas (Faceted)
cell_biology,developmental_biology,immunology,infectious_disease,neurobiology,signal_transduction
Species
human
Target Sentence
WW domain-containing protein 1 (WWP1) functions as an E3 ubiquitin-protein ligase. WWP1 ubiquitinates ERBB4 isoforms JM-A CYT-1 and JM-B CYT-1, KLF2, KLF5 and TP63 and promotes their proteasomal degradation. WWP1 also ubiquitinates and promotes degradation of TGFBR1; TGFBR1 ubiquitination is enhanced by SMAD7, and WWP1 also ubiquitinates SMAD7 as well as SMAD6. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9H0M0].
UniGene ID
Hs.655189
UniProt ID (eCommerce)
Q9H0M0

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.