human VRK1 shRNA silencing Adenovirus

Name 
Ad-h-VRK1-shRNA
Cat No 
shADV-227553
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human VRK1.

shADV-227553
Ad-h-VRK1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human VRK1 (vaccinia related kinase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
PCH1; PCH1A; serine/threonine-protein kinase VRK1; vaccinia related kinase 1; vaccinia virus B1R-related kinase 1; Vaccinia-related kinase 1
Description (eCommerce)
Vaccinia-related kinase 1 is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases.VRK1 is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This protein, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN [taken from NCBI Entrez Gene (Gene ID: 7443)].
Description (Vector)
VRK1 encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. VRK1 is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN.
Gene ID
7443
Gene Name (eCommerce)
VRK serine/threonine kinase 1
Gene Name (Vector)
vaccinia related kinase 1
Gene Symbol
VRK1
HGNC ID
HGNC:12718
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1191
Protein Name (eCommerce)
Serine/threonine-protein kinase VRK1
RefSeq ID
NM_003384
RefSeq Synonyms
NM_003384, BC113510, BC112075, BC103761,
Research Areas
Cell Cycle,Cell Division,Cytoskeleton,Golgi Apparatus,Kinase/Phosphatase
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,signal_transduction
Species
human
Target Sentence
Vaccinia-related kinase 1 is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases.VRK1 is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This protein, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN [taken from NCBI Entrez Gene (Gene ID: 7443)].
UniGene ID
Hs.422662
UniProt ID (eCommerce)
Q99986

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.