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human UPF3B shRNA silencing Adenovirus

Name 
Ad-h-UPF3B-shRNA
Cat No 
shADV-227227
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human UPF3B.

shADV-227227
Ad-h-UPF3B-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human UPF3B (UPF3 regulator of nonsense transcripts homolog B (yeast)). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
HUPF3B; hUpf3p-X; mental retardation, X-linked 62; MRX62; MRXS14; nonsense mRNA reducing factor 3B; regulator of nonsense transcripts 3B; RENT3B; UPF3 regulator of nonsense transcripts homolog B; UPF3B pseudogene 1; UPF3B pseudogene 2; UPF3B pseudogene 3; UPF3BP1; UPF3BP2; UPF3BP3; Upf3p-X; UPF3X; up-frameshift suppressor 3 homolog B; up-frameshift suppressor 3 homolog on chromosome X
Description (eCommerce)
UPF3B (UPF regulator of nonsense transcripts homolog B) is involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. UPF3B recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF2, UPF3B stimulates both ATPase and RNA helicase activities of UPF1 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9BZI7].
Description (Vector)
UPF3B encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. UPF3B is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene.
Gene ID
65109
Gene Name (eCommerce)
UPF3B, regulator of nonsense mediated mRNA decay
Gene Name (Vector)
UPF3 regulator of nonsense transcripts homolog B (yeast)
Gene Symbol
UPF3B
HGNC ID
HGNC:20439
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1413
ORF Size (bp)
1413 bp
Protein Name (eCommerce)
Regulator of nonsense transcripts 3B
RefSeq ID
NM_023010
RefSeq Synonyms
NM_080632, NM_023010, BC121017,
Research Areas
Gene Expression,mRNA Binding,mRNA Processing,mRNA Splicing,mRNA Transport,Neurobiology,RNA Binding,RNA Processing,RNA Transport,Spliceosome,Translational Control,Transport
Research Areas (Faceted)
neurobiology,genetics,transcription_translation,cell_biology
Species
human
Target Sentence
UPF3B (UPF regulator of nonsense transcripts homolog B) is involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. UPF3B recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF2, UPF3B stimulates both ATPase and RNA helicase activities of UPF1 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9BZI7].
UniGene ID
Hs.103832
UniProt ID (eCommerce)
Q9BZI7

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.