human MAP3K3 Over-expression AAV

Name 
AAV-h-MAP3K3
Cat No 
AAV-214930
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human MAP3K3.

AAV-214930
AAV-h-MAP3K3

Ready-to-use AAV expressing Human MAP3K3 (mitogen-activated protein kinase kinase kinase 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
MAP/ERK kinase kinase 3; MAPK/ERK kinase kinase 3; MAPKKK3; MEK kinase 3; MEKK 3; MEKK3; mitogen-activated protein kinase kinase kinase 3
Description (eCommerce)
MEK (MAPK/ERK kinase) kinase 3 (MEKK3) is a component of a protein kinase signal transduction cascade. MEKK3 has been shown to mediate activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q99759].
Description (Vector)
MAP3K3 product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Gene ID
4215
Gene Name (eCommerce)
mitogen-activated protein kinase kinase kinase 3
Gene Name (Vector)
mitogen-activated protein kinase kinase kinase 3
Gene Symbol
MAP3K3
HGNC ID
HGNC:6855
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1881
ORF Size (bp)
1881 bp
Protein Name (eCommerce)
Mitogen-activated protein kinase kinase kinase 3
RefSeq ID
NM_002401
RefSeq Synonyms
NM_203351, NM_002401, NM_001330431, BC093674, BC093672, BC090859, BC010464,
Research Areas
Cytokine Signaling,EGFR Signaling,EPO Signaling,GnRH Signaling,Host-Virus Interactions,Immunology,Insulin Signaling,Interleukin Signaling,JAK-STAT Signaling,Kinase/Phosphatase,MAPK Signaling,Neurobiology,NF-kB Signaling,Signal Transduction,TNF Signaling,VEGF Signaling
Research Areas (Faceted)
cell_biology,immunology,infectious_disease,neurobiology,signal_transduction
Species
human
Target Sentence
MEK (MAPK/ERK kinase) kinase 3 (MEKK3) is a component of a protein kinase signal transduction cascade. MEKK3 has been shown to mediate activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q99759].
UniGene ID
Hs.29282
UniProt ID (eCommerce)
Q99759

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.