human SPDEF shRNA silencing Adenovirus

Name 
Ad-h-SPDEF-shRNA
Cat No 
shADV-224142
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human SPDEF.

shADV-224142
Ad-h-SPDEF-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human SPDEF (SAM pointed domain containing ETS transcription factor). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
bA375E1.3; PDEF
Description (Vector)
SPDEF belongs to the ETS family of transcription factors. It is highly expressed in the prostate epithelial cells, and functions as an androgen-independent transactivator of prostate-specific antigen (PSA) promoter. Higher expression of this protein has also been reported in brain, breast, lung and ovarian tumors, compared to the corresponding normal tissues, and it shows better tumor-association than other cancer-associated molecules, making it a more suitable target for developing specific cancer therapies. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Gene ID
25803
Gene Name (Vector)
SAM pointed domain containing ETS transcription factor
Gene Symbol
SPDEF
HGNC ID
HGNC:17257
ORF Size (aa)
1008
RefSeq ID
NM_012391
RefSeq Synonyms
NM_012391, NM_001252294, BC021299,
Species
human
UniGene ID
Hs.485158

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.