human RGS14 shRNA silencing Adenovirus

Name 
Ad-h-RGS14-shRNA
Cat No 
shADV-221114
Availability
7-8 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human RGS14.

shADV-221114
Ad-h-RGS14-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human RGS14 (regulator of G-protein signaling 14). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
regulator of G-protein signaling 14; regulator of G-protein signalling 14; RGS14
Description (eCommerce)
Regulator of G-protein signaling 14 (RGS14) acts as a regulator of G protein signaling (RGS). Modulates G protein alpha subunits nucleotide exchange and hydrolysis activities by functioning either as a GTPase-activating protein (GAP), thereby driving G protein alpha subunits into their inactive GDP-bound form, or as a GDP-dissociation inhibitor (GDI). RGS14 may act as a scaffold integrating G protein and Ras/Raf MAPkinase signaling pathways [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O43566].
Description (Vector)
RGS14 encodes a member of the regulator of G-protein signaling family. This protein contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
Gene ID
10636
Gene Name (eCommerce)
regulator of G protein signaling 14
Gene Name (Vector)
regulator of G-protein signaling 14
Gene Symbol
RGS14
HGNC ID
HGNC:9996
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1701
ORF Size (bp)
1701 bp
Protein Name (eCommerce)
Regulator of G-protein signaling 14
RefSeq ID
NM_006480
RefSeq Synonyms
NM_006480, BC014094,
Research Areas
Cardiology,Cardiovascular,Cell Cycle,Cell Division,Cytoskeleton,GPCR Signaling,Neurobiology,Neurodevelopment,Neurogenesis,Signal Transduction,Synapse
Research Areas (Faceted)
cardiovascular,cell_biology,cell_cycle,developmental_biology,genetics,neurobiology,signal_transduction
Species
human
Target Sentence
Regulator of G-protein signaling 14 (RGS14) acts as a regulator of G protein signaling (RGS). Modulates G protein alpha subunits nucleotide exchange and hydrolysis activities by functioning either as a GTPase-activating protein (GAP), thereby driving G protein alpha subunits into their inactive GDP-bound form, or as a GDP-dissociation inhibitor (GDI). RGS14 may act as a scaffold integrating G protein and Ras/Raf MAPkinase signaling pathways [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O43566].
UniGene ID
Hs.9347
UniProt ID (eCommerce)
O43566

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.