human RC3H1 shRNA silencing Adenovirus

Name 
Ad-h-RC3H1-shRNA
Cat No 
shADV-220930
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human RC3H1.

shADV-220930
Ad-h-RC3H1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human RC3H1 (ring finger and CCCH-type domains 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
probable E3 ubiquitin-protein ligase Roquin; RING finger and C3H zinc finger protein 1; RING finger and CCCH-type zinc finger domain-containing protein 1; RING finger protein 198; RNF198; ROQUIN; roquin-1
Description (eCommerce)
The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes and the ligation of ubiquitin to the target protein by E3 enzymes. Roquin is a member of the RING type E3 ubiquitin ligases. Roquin possesses a CCCH zinc-finger found in RNA-binding proteins and is implicated to play a role in the development of autoimmune disease.
Description (Vector)
RC3H1, or roquin, encodes a highly conserved member of the RING type ubiquitin ligase protein family (Vinuesa et al., 2005 (PubMed 15917799)). The roquin protein is distinguished by the presence of a CCCH zinc finger found in RNA-binding proteins, and localization to cytosolic RNA granules implicated in regulating mRNA translation and stability.(supplied by OMIM, Mar 2008)
Gene ID
149041
Gene Name (eCommerce)
ring finger and CCCH-type domains 1
Gene Name (Vector)
ring finger and CCCH-type domains 1
Gene Symbol
RC3H1
HGNC ID
HGNC:29434
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3405
ORF Size (bp)
3405 bp
Protein Name (eCommerce)
Roquin-1
RefSeq ID
NM_172071
RefSeq Synonyms
NM_172071, NM_001300852, NM_001300851, NM_001300850, BC144408, BC136784,
Research Areas
Immunology,mRNA Binding,RNA Binding,RNA Processing,T-cell Receptor Signaling,Ubiquitination,Zinc-finger
Research Areas (Faceted)
cell_biology,genetics,transcription_translation,immunology,signal_transduction
Species
human
Target Sentence
The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes and the ligation of ubiquitin to the target protein by E3 enzymes. Roquin is a member of the RING type E3 ubiquitin ligases. Roquin possesses a CCCH zinc-finger found in RNA-binding proteins and is implicated to play a role in the development of autoimmune disease.
UniGene ID
Hs.30258
UniProt ID (eCommerce)
Q5TC82

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.