human RASA1 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Human RASA1.
shADV-220757
Ad-h-RASA1-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Human RASA1 (RAS p21 protein activator 1). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
CMAVM; CM-AVM; GAP; GTPase-activating protein; p120; p120 RAS GTPase activating protein; p120GAP; p120RASGAP; PKWS; ras GTPase-activating protein 1; Ras p21 protein activator; RAS p21 protein activator (GTPase activating protein) 1; RASA; RASGAP; triphosphatase-activating protein
Description (eCommerce)
RASA1 is an inhibitory regulator of the Ras-cyclic AMP pathway. It Stimulates the GTPase of normal but not oncogenic Ras p21; this stimulation may be further increased in the presence of NCK1Â [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P20936].
Description (Vector)
RASA1 is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues.
Gene ID
5921
Gene Name (eCommerce)
RAS p21 protein activator 1
Gene Name (Vector)
RAS p21 protein activator 1
Gene Symbol
RASA1
HGNC ID
HGNC:9871
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3144
ORF Size (bp)
3144 bp
Protein Name (eCommerce)
Ras GTPase-activating protein 1
RefSeq ID
NM_022650
RefSeq Synonyms
NM_022650, NM_002890, BC033015,
Research Areas
Apoptosis,Axon Guidance,B-cell Receptor Signaling,Cancer,Cardiovascular,EGFR Signaling,EPO Signaling,Fc Receptor Signaling,HGF Signaling,Host-Virus Interactions,IGF-1 Signaling,Immunology,Insulin Signaling,Interleukin Signaling,MAPK Signaling,Neurobiology,Neurodevelopment,PDGF Signaling,Prostate Cancer,RNA Processing,SCF-KIT Signaling,Signal Transduction,T-cell Receptor Signaling,TPO Signaling,VEGF Signaling
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,developmental_biology,genetics,immunology,infectious_disease,neurobiology,signal_transduction,stem_cell_biology,transcription_translation
Species
human
Target Sentence
RASA1 is an inhibitory regulator of the Ras-cyclic AMP pathway. It Stimulates the GTPase of normal but not oncogenic Ras p21; this stimulation may be further increased in the presence of NCK1Â [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P20936].
UniGene ID
Hs.664080
UniProt ID (eCommerce)
P20936
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.