human PURA shRNA silencing Adenovirus

Name 
Ad-h-PURA-shRNA
Cat No 
shADV-220396
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human PURA.

shADV-220396
Ad-h-PURA-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PURA (purine rich element binding protein A). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
MRD31; PUR1; PURALPHA; PUR-ALPHA; purine-rich single-stranded DNA-binding protein alpha; transcriptional activator protein Pur-alpha
Description (eCommerce)
Transcriptional activator protein pur-alpha (purine-rich single stranded DNA-binding protein alpha) is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene. Pur-alpha may play a role in the initiation of DNA replication and in recombination [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q00577].
Description (Vector)
PURA product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia.
Gene ID
5813
Gene Name (eCommerce)
purine rich element binding protein A
Gene Name (Vector)
purine rich element binding protein A
Gene Symbol
PURA
HGNC ID
HGNC:9701
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
969
Protein Name (eCommerce)
Transcriptional activator protein Pur-alpha
RefSeq ID
NM_005859
RefSeq Synonyms
NM_005859, BC036087,
Research Areas
Cell Cycle,DNA Replication,Immunology,Neurobiology,Neurodevelopment,Replication,Transcription Factor/Regulator,Translational Control
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,transcription_translation,immunology,neurobiology,developmental_biology,cell_biology,cell_cycle,genetics,transcription_translation
Species
human
Target Sentence
Transcriptional activator protein pur-alpha (purine-rich single stranded DNA-binding protein alpha) is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene. Pur-alpha may play a role in the initiation of DNA replication and in recombination [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q00577].
UniGene ID
Hs.443121
UniProt ID (eCommerce)
Q00577

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.