human PUF60 shRNA silencing Adenovirus

Name 
Ad-h-PUF60-shRNA
Cat No 
shADV-220390
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human PUF60.

shADV-220390
Ad-h-PUF60-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PUF60 (poly(U) binding splicing factor 60). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
60 kDa poly(U)-binding-splicing factor; FBP interacting repressor; FBP-interacting repressor; FIR; FUSE-binding protein-interacting repressor; poly(U) binding splicing factor 60KDa; poly(U)-binding-splicing factor PUF60; pyrimidine tract binding splicing factor; Ro ribonucleoprotein-binding protein 1; Ro-binding protein 1; RoBP1; RoBPI; Siah binding protein 1; Siah-binding protein 1; SIAHBP1; Siah-BP1; VRJS
Description (eCommerce)
Poly-U binding splicing factor 60KDa (PUF60) is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. PUF60 is implicated in the xeroderma pigmentosum disorder [taken from NCBI Entrez Gene (Gene ID: 22827)].
Description (Vector)
PUF60 encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Gene ID
22827
Gene Name (eCommerce)
poly(U) binding splicing factor 60
Gene Name (Vector)
poly(U) binding splicing factor 60
Gene Symbol
PUF60
HGNC ID
HGNC:17042
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1551
ORF Size (bp)
1551 bp
Protein Name (eCommerce)
Poly(U)-binding-splicing factor PUF60
RefSeq ID
NM_014281
RefSeq Synonyms
NM_078480, NM_014281, NM_001271100, NM_001271099, NM_001271098, NM_001271097, NM_001271096, NM_001136033, BC011979, BC011265, BC009734, BC008875,
Research Areas
Apoptosis,Gene Expression,mRNA Processing,mRNA Splicing,RNA Binding,RNA Processing,Spliceosome,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,genetics,transcription_translation
Species
human
Target Sentence
Poly-U binding splicing factor 60KDa (PUF60) is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. PUF60 is implicated in the xeroderma pigmentosum disorder [taken from NCBI Entrez Gene (Gene ID: 22827)].
UniGene ID
Hs.521924
UniProt ID (eCommerce)
Q9UHX1

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.