human PTRH2 shRNA silencing Adenovirus

Name 
Ad-h-PTRH2-shRNA
Cat No 
shADV-220370
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human PTRH2.

shADV-220370
Ad-h-PTRH2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PTRH2 (peptidyl-tRNA hydrolase 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
bcl-2 inhibitor of transcription 1; BIT1; CFAP37; CGI-147; cilia and flagella associated protein 37; IMNEPD; peptidyl-tRNA hydrolase 2, mitochondrial; PTH; PTH 2; PTH2
Description (eCommerce)
Peptidyl-tRNA hydrolase 2 (PTRH2) is a mitochondrial protein with two putative domains, an N-terminal mitochondrial localization sequence, and a UPF0099 domain. In vitro assays suggest that this protein possesses peptidyl-tRNA hydrolase activity, to release the peptidyl moiety from tRNA, thereby preventing the accumulation of dissociated peptidyl-tRNA that could reduce the efficiency of translation. This protein also plays a role regulating cell survival and death. It promotes survival as part of an integrin-signaling pathway for cells attached to the extracellular matrix (ECM), but also promotes apoptosis in cells that have lost their attachment to the ECM, a process called anoikos [taken from NCBI Entrez Gene (Gene ID: 51651)].
Gene ID
51651
Gene Name (eCommerce)
peptidyl-tRNA hydrolase 2
Gene Name (Vector)
peptidyl-tRNA hydrolase 2
Gene Symbol
PTRH2
HGNC ID
HGNC:24265
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
540
ORF Size (bp)
540 bp
Protein Name (eCommerce)
Peptidyl-tRNA hydrolase 2, mitochondrial
RefSeq ID
NM_016077
RefSeq Synonyms
NM_016077, NM_001015509, BC006807,
Research Areas
Apoptosis,Gene Expression,Mitochondrion
Research Areas (Faceted)
cell_biology
Species
human
Target Sentence
Peptidyl-tRNA hydrolase 2 (PTRH2) is a mitochondrial protein with two putative domains, an N-terminal mitochondrial localization sequence, and a UPF0099 domain. In vitro assays suggest that this protein possesses peptidyl-tRNA hydrolase activity, to release the peptidyl moiety from tRNA, thereby preventing the accumulation of dissociated peptidyl-tRNA that could reduce the efficiency of translation. This protein also plays a role regulating cell survival and death. It promotes survival as part of an integrin-signaling pathway for cells attached to the extracellular matrix (ECM), but also promotes apoptosis in cells that have lost their attachment to the ECM, a process called anoikos [taken from NCBI Entrez Gene (Gene ID: 51651)].
UniGene ID
Hs.12677
UniProt ID (eCommerce)
Q9Y3E5

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.