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human PRKCH shRNA silencing Adenovirus

Name 
Ad-h-PRKCH-shRNA
Cat No 
shADV-219865
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human PRKCH.

shADV-219865
Ad-h-PRKCH-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PRKCH (protein kinase C eta). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
nPKC-eta; PKCL; PKC-L; PRKCL; protein kinase C eta type
Description (eCommerce)
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC-eta is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. PKC-eta can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene [taken from NCBI Entrez Gene (Gene ID: 5583)].
Description (Vector)
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene.
Gene ID
5583
Gene Name (eCommerce)
protein kinase C eta
Gene Name (Vector)
protein kinase C eta
Gene Symbol
PRKCH
HGNC ID
HGNC:9403
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2052
ORF Size (bp)
2052 bp
Protein Name (eCommerce)
Protein kinase C eta type
RefSeq ID
NM_006255
RefSeq Synonyms
NM_006255, BC037268, BC001000,
Research Areas
Apoptosis,Cancer,Cardiology,Cardiovascular,EPO Signaling,Glioma,GPCR Signaling,Immunology,Insulin Signaling,Interleukin Signaling,JAK-STAT Signaling,Kinase/Phosphatase,MAPK Signaling,Neurobiology,Neurodevelopment,NFAT Signaling,Signal Transduction,VEGF Signaling,Wnt Signaling,Zinc-finger
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,developmental_biology,immunology,neurobiology,signal_transduction
Species
human
Target Sentence
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC-eta is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. PKC-eta can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene [taken from NCBI Entrez Gene (Gene ID: 5583)].
UniGene ID
Hs.333907
UniProt ID (eCommerce)
P24723

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.