human PRKAB1 shRNA silencing Adenovirus

Name 
Ad-h-PRKAB1-shRNA
Cat No 
shADV-219835
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human PRKAB1.

shADV-219835
Ad-h-PRKAB1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PRKAB1 (protein kinase AMP-activated non-catalytic subunit beta 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
AMPK; HAMPKb
Description (Vector)
PRKAB1 is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.
Gene ID
5564
Gene Name (Vector)
protein kinase AMP-activated non-catalytic subunit beta 1
Gene Symbol
PRKAB1
HGNC ID
HGNC:9378
ORF Size (aa)
813
RefSeq ID
NM_006253
RefSeq Synonyms
NM_006253, BC017671, BC001823, BC001056, BC001007,
Species
human
UniGene ID
Hs.715515

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.