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human POLD2 shRNA silencing Adenovirus

Name 
Ad-h-POLD2-shRNA
Cat No 
shADV-219348
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human POLD2.

shADV-219348
Ad-h-POLD2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human POLD2 (DNA polymerase delta 2, accessory subunit). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
DAN polymerase delta 2, accessory subunit; DNA polymerase delta subunit 2; DNA polymerase delta subunit p50; Pol delta B subunit (p50); polymerase (DNA directed), delta 2, accessory subunit; polymerase (DNA directed), delta 2, regulatory subunit 50kDa; polymerase (DNA) delta 2, accessory subunit
Description (eCommerce)
DNA polymerase delta subunit 2 (PolD2) is the 50-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. PolD2 is required for the stimulation of DNA polymerase delta activity by the processivity cofactor proliferating cell nuclear antigen (PCNA [taken from NCBI Entrez Gene (Gene ID: 5425)].
Description (Vector)
POLD2 encodes the 50-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein is required for the stimulation of DNA polymerase delta activity by the processivity cofactor proliferating cell nuclear antigen (PCNA). Expression of this gene may be a marker for ovarian carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5.
Gene ID
5425
Gene Name (eCommerce)
DNA polymerase delta 2, accessory subunit
Gene Name (Vector)
DNA polymerase delta 2, accessory subunit
Gene Symbol
POLD2
HGNC ID
HGNC:9176
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1410
ORF Size (bp)
1410 bp
Protein Name (eCommerce)
DNA polymerase delta subunit 2
RefSeq ID
NM_006230
RefSeq Synonyms
NM_006230, NM_001256879, NM_001127218, BC000459,
Research Areas
Cell Cycle,DNA Damage/Repair,DNA Replication,Homologous Recombination,Host-Virus Interactions,Immunology,Replication,Telomeric Maintenance
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,transcription_translation,infectious_disease,immunology
Species
human
Target Sentence
DNA polymerase delta subunit 2 (PolD2) is the 50-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. PolD2 is required for the stimulation of DNA polymerase delta activity by the processivity cofactor proliferating cell nuclear antigen (PCNA [taken from NCBI Entrez Gene (Gene ID: 5425)].
UniGene ID
Hs.306791
UniProt ID (eCommerce)
P49005

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.