human LPIN1 shRNA silencing Adenovirus

Name 
Ad-h-LPIN1-shRNA
Cat No 
shADV-214456
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human LPIN1.

shADV-214456
Ad-h-LPIN1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human LPIN1 (lipin 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
Lipin-1; PAP1; phosphatidate phosphatase LPIN1
Description (eCommerce)
LPIN1 is a magnesium-ion-dependent phosphatidic acid phosphohydrolase enzyme that catalyzes the penultimate step in triglyceride synthesis including the dephosphorylation of phosphatidic acid to yield diacylglycerol. LPIN1 is required for adipocyte differentiation and it also functions as a nuclear transcriptional coactivator with some peroxisome proliferator-activated receptors to modulate expression of other genes involved in lipid metabolis [taken from NCBI Entrez Gene (Gene ID: 23175)].
Description (Vector)
LPIN1 encodes a magnesium-ion-dependent phosphatidic acid phosphohydrolase enzyme that catalyzes the penultimate step in triglyceride synthesis including the dephosphorylation of phosphatidic acid to yield diacylglycerol. Expression of this gene is required for adipocyte differentiation and it also functions as a nuclear transcriptional coactivator with some peroxisome proliferator-activated receptors to modulate expression of other genes involved in lipid metabolism. Mutations in this gene are associated with metabolic syndrome, type 2 diabetes, and autosomal recessive acute recurrent myoglobinuria (ARARM). LPIN1 is also a candidate for several human lipodystrophy syndromes. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their full-length structures have not been determined.
Gene ID
23175
Gene Name (eCommerce)
lipin 1
Gene Name (Vector)
lipin 1
Gene Symbol
LPIN1
HGNC ID
HGNC:13345
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2673
ORF Size (bp)
2673 bp
Protein Name (eCommerce)
Phosphatidate phosphatase LPIN1
RefSeq ID
NM_145693
RefSeq Synonyms
NM_145693, NM_001261429, NM_001261428, NM_001261427, BC030537,
Research Areas
Cytoskeleton,Histone Modification,Metabolism/Metabolic Process,RNA Binding,Signal Transduction,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,metabolism,signal_transduction,transcription_translation
Species
human
Target Sentence
LPIN1 is a magnesium-ion-dependent phosphatidic acid phosphohydrolase enzyme that catalyzes the penultimate step in triglyceride synthesis including the dephosphorylation of phosphatidic acid to yield diacylglycerol. LPIN1 is required for adipocyte differentiation and it also functions as a nuclear transcriptional coactivator with some peroxisome proliferator-activated receptors to modulate expression of other genes involved in lipid metabolis [taken from NCBI Entrez Gene (Gene ID: 23175)].
UniGene ID
Hs.467740
UniProt ID (eCommerce)
Q14693

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.