human DDX56 shRNA silencing Adenovirus

Name 
Ad-h-DDX56-shRNA
Cat No 
shADV-206773
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human DDX56.

shADV-206773
Ad-h-DDX56-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human DDX56 (DEAD-box helicase 56). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
61-kd nucleolar helicase; ATP-dependent 61 kDa nucleolar RNA helicase; DDX21; DDX26; DEAD (Asp-Glu-Ala-Asp) box helicase 56; DEAD (Asp-Glu-Ala-Asp) box polypeptide 56; DEAD box protein 21; DEAD box protein 56; DEAD-box RNA helicase; NOH61; nucleolar helicase of 61 kDa; probable ATP-dependent RNA helicase DDX56; putative nucleolar RNA helicase
Description (eCommerce)
DDX56 (DEAD box polypeptide 56) is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX56 is propososed to be involved in ribosome biosynthesis and the assembly of the large (60S) ribosomal subunit.
Description (Vector)
DDX56 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene shows ATPase activity in the presence of polynucleotides and associates with nucleoplasmic 65S preribosomal particles. DDX56 may be involved in ribosome synthesis, most likely during assembly of the large 60S ribosomal subunit. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene ID
54606
Gene Name (eCommerce)
DEAD-box helicase 56
Gene Name (Vector)
DEAD-box helicase 56
Gene Symbol
DDX56
HGNC ID
HGNC:18193
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1644
ORF Size (bp)
1644 bp
Protein Name (eCommerce)
Probable ATP-dependent RNA helicase DDX56
RefSeq ID
NM_019082
RefSeq Synonyms
NM_019082, NM_001257189, BC001235,
Research Areas
Helicase Activity,RNA Binding,RNA Processing
Research Areas (Faceted)
cell_biology,genetics,transcription_translation
Species
human
Target Sentence
DDX56 (DEAD box polypeptide 56) is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX56 is propososed to be involved in ribosome biosynthesis and the assembly of the large (60S) ribosomal subunit.
UniGene ID
Hs.654762
UniProt ID (eCommerce)
Q9NY93

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.