human CDC27 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Human CDC27.
shADV-204744
Ad-h-CDC27-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Human CDC27 (cell division cycle 27). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
ANAPC3; anaphase promoting complex subunit 3; Anaphase-promoting complex subunit 3; anaphase-promoting complex, protein 3; APC3; CDC27 homolog; CDC27Hs; cell division cycle 27 homolog; cell division cycle protein 27 homolog; D0S1430E; D0S1430E, D17S978E; D17S978E; HNUC; H-NUC; NUC2; nuc2 homolog
Description (eCommerce)
CDC27 is a component of the APC/c (anaphase promoting complex/cyclosome) which is responsible for the ubiquitination and degradation of securin and cyclin B that prompts the onset of anaphase and exit from mitosis. The APC/c is composed of at least 11 subunits. Three subunits, CDC27 CDC16 and CDC23, contain a TPR (tetratricopeptide repeat) important for protein-protein interactions. CDC27 has been shown to interact with mitotic checkpoint proteins such as Mad2, p55CDC and BUBR1.
Description (Vector)
CDC27 shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Gene ID
996
Gene Name (eCommerce)
cell division cycle 27
Gene Name (Vector)
cell division cycle 27
Gene Symbol
CDC27
HGNC ID
HGNC:1728
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2493
ORF Size (bp)
2493 bp
Protein Name (eCommerce)
Cell division cycle protein 27 homolog
RefSeq ID
NM_001256
RefSeq Synonyms
NM_001293091, NM_001293089, NM_001256, NM_001114091, BC011656,
Research Areas
Antigen Processing and Presentation,Cell Cycle,Cell Division,Host-Virus Interactions,Immunology,Meiosis,Signal Transduction,TGF-beta Signaling
Research Areas (Faceted)
cell_biology,cell_cycle,developmental_biology,genetics,immunology,infectious_disease,signal_transduction
Species
human
Target Sentence
CDC27 is a component of the APC/c (anaphase promoting complex/cyclosome) which is responsible for the ubiquitination and degradation of securin and cyclin B that prompts the onset of anaphase and exit from mitosis. The APC/c is composed of at least 11 subunits. Three subunits, CDC27 CDC16 and CDC23, contain a TPR (tetratricopeptide repeat) important for protein-protein interactions. CDC27 has been shown to interact with mitotic checkpoint proteins such as Mad2, p55CDC and BUBR1.
UniGene ID
Hs.463295
UniProt ID (eCommerce)
P30260
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.