human CASP9 shRNA silencing Adenovirus

Name 
Ad-h-CASP9-shRNA
Cat No 
shADV-204127
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human CASP9.

shADV-204127
Ad-h-CASP9-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human CASP9 (caspase 9). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
APAF3; APAF-3; apoptotic protease activating factor 3; apoptotic protease MCH-6; Apoptotic protease-activating factor 3; CASP-9; caspase 9, apoptosis-related cysteine peptidase; caspase-9; ICE-LAP6; ICE-like apoptotic protease 6; MCH6; PPP1R56; protein phosphatase 1, regulatory subunit 56
Description (eCommerce)
Caspase 9 is involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P55211].
Description (Vector)
CASP9 encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms.
Gene ID
842
Gene Name (eCommerce)
caspase 9
Gene Name (Vector)
caspase 9
Gene Symbol
CASP9
HGNC ID
HGNC:1511
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1251
ORF Size (bp)
1251 bp
Protein Name (eCommerce)
Caspase-9
RefSeq ID
NM_001229
RefSeq Synonyms
NM_032996, NM_001278054, NM_001229, BC006463, BC002452,
Research Areas
Alzheimer's Disease,Amyotrophic Lateral Sclerosis (ALS),Apoptosis,B-cell Receptor Signaling,Breast Cancer,Cancer,Cardiology,Cardiovascular,CD95/FAS Signaling,Cell Cycle,Colorectal Cancer,DNA Damage/Repair,EGFR Signaling,EGFR Signaling in Cancer,Fc Receptor Signaling,FGF Signaling,Host-Virus Interactions,Huntington's Disease,Immunology,Innate Immunity,Kinase/Phosphatase,Lung Cancer,MAPK Signaling,Neurobiology,Neurodegeneration,Neurodevelopment,NGF Signaling,p53 Signaling,Parkinson's Disease,PDGF Signaling,PI3K/Akt Signaling,PI3K-AKT Signaling in Cancer,Prostate Cancer,SCF-KIT Signaling,Signal Transduction,TNF Signaling,Toll-like Receptor Signaling,VEGF Signaling
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,cell_cycle,developmental_biology,genetics,immunology,infectious_disease,neurobiology,signal_transduction,stem_cell_biology,transcription_translation
Species
human
Target Sentence
Caspase 9 is involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P55211].
UniGene ID
Hs.329502
UniProt ID (eCommerce)
P55211

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.