human ATP5H shRNA silencing Adenovirus

Name 
Ad-h-ATP5H-shRNA
Cat No 
shADV-201726
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human ATP5H.

shADV-201726
Ad-h-ATP5H-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human ATP5H (ATP synthase, H+ transporting, mitochondrial Fo complex subunit D). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
APT5H; ATP synthase D chain, mitochondrial; ATP synthase peripheral stalk subunit d; ATP synthase subunit d, mitochondrial; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d; ATP synthase, H+ transporting, mitochondrial F1F0, subunit d; ATP synthase, H+ transporting, mitochondrial Fo complex subunit D; ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d; ATP5H; ATPase subunit d; ATPQ; My032 protein
Description (eCommerce)
Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5H is the d subunit of the Fo complex [taken from NCBI Entrez Gene (Gene ID: 10476)].
Description (Vector)
Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15.
Gene ID
10476
Gene Name (eCommerce)
ATP synthase peripheral stalk subunit d
Gene Name (Vector)
ATP synthase, H+ transporting, mitochondrial Fo complex subunit D
Gene Symbol
ATP5H
HGNC ID
HGNC:845
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
414
Protein Name (eCommerce)
ATP synthase subunit d, mitochondrial
RefSeq ID
NM_006356
RefSeq Synonyms
NM_006356, NM_001003785, BC038092, BC032245,
Research Areas
Alzheimer's Disease,Cancer,Cardiology,Cell Biology,Huntington's Disease,Metabolism/Metabolic Process,Mitochondrion,Neurobiology,Neurodegeneration,Parkinson's Disease,Transport
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,metabolism,neurobiology
Species
human
Target Sentence
Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5H is the d subunit of the Fo complex [taken from NCBI Entrez Gene (Gene ID: 10476)].
UniGene ID
Hs.514465
UniProt ID (eCommerce)
O75947

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.