human ATG10 shRNA silencing Adenovirus

Name 
Ad-h-ATG10-shRNA
Cat No 
shADV-201632
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human ATG10.

shADV-201632
Ad-h-ATG10-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human ATG10 (autophagy related 10). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
60
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
APG10; APG10L; pp12616
Description (Vector)
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 (PubMed 12890687)).(supplied by OMIM, Mar 2008)
Gene ID
83734
Gene Name (Vector)
autophagy related 10
Gene Symbol
ATG10
HGNC ID
HGNC:20315
ORF Size (aa)
663
ORF Size (bp)
663 bp
RefSeq ID
NM_031482
RefSeq Synonyms
NM_031482, NM_001131028, BC029268, BC004551,
Species
human
UniGene ID
Hs.713698

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About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.