human ADD3 shRNA silencing Adenovirus

Name
Ad-h-ADD3-shRNA

Cat No
shADV-200444

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human ADD3.

shADV-200444

Ad-h-ADD3-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human ADD3 (adducin 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

ADDL; adducin 3 (gamma); adducin-like protein 70; CPSQ3; gamma-adducin

Description (eCommerce)

Gamma-adducin is a member of the adducin family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Originally purified from human erythrocytes, adducin was found to be a heterodimer of adducins alpha and beta. Heterodimers consisting of alpha and gamma subunits have also been described [taken from NCBI Entrez Gene (Gene ID: 120)].

Description (Vector)

Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known.

Gene ID

120

Gene Name (eCommerce)

adducin 3

Gene Name (Vector)

adducin 3

Gene Symbol

ADD3

HGNC ID

HGNC:245

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

2121

ORF Size (bp)

2121 bp

Protein Name (eCommerce)

Gamma-adducin

RefSeq ID

NM_019903

RefSeq Synonyms

NM_019903, NM_016824, NM_001320594, NM_001320593, NM_001320592, NM_001320591, NM_001121, BC062559,

Research Areas

Cytoskeleton

Research Areas (Faceted)

cell_biology

Species

human

Target Sentence

UniGene ID

Hs.501012

UniProt ID (eCommerce)

Q9UEY8

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.