rat UPF3A shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Rat Upf3a.
shAAV-293034
AAV-r-UPF3A-shRNA
Ready-to-use AAV expressing shRNA for silencing of Rat Upf3a (UPF3 regulator of nonsense transcripts homolog A (yeast)). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Description (Vector)
Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. However, UPF3A is shown to be only marginally active in NMD as compared to UPF3B. Binds spliced mRNA upstream of exon-exon junctions. In vitro, weakly stimulates translation.
Gene ID
361176
Gene Name (Vector)
UPF3 regulator of nonsense transcripts homolog A (yeast)
Gene Symbol
Upf3a
ORF Size (aa)
1218
ORF Size (bp)
1218 bp
RefSeq ID
NM_001012159
RefSeq Synonyms
NM_001012159, BC088463,
Species
rat
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.