rat XRCC6 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Rat Xrcc6.
shAAV-291916
AAV-r-XRCC6-shRNA
Ready-to-use AAV expressing shRNA for silencing of Rat Xrcc6 (X-ray repair cross complementing 6). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
G22p1; Ku70; Kup70
Description (Vector)
Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
Gene ID
25019
Gene Name (Vector)
X-ray repair cross complementing 6
Gene Symbol
Xrcc6
ORF Size (aa)
1827
ORF Size (bp)
1827 bp
RefSeq ID
NM_139080
RefSeq Synonyms
NM_139080, BC078718,
Species
rat
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.