rat PPP2CA shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Rat Ppp2ca.
shAAV-290648
AAV-r-PPP2CA-shRNA
Ready-to-use AAV expressing shRNA for silencing of Rat Ppp2ca (protein phosphatase 2, catalytic subunit, alpha isozyme). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
Pp2a1
Description (Vector)
PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'.
Gene ID
24672
Gene Name (Vector)
protein phosphatase 2, catalytic subunit, alpha isozyme
Gene Symbol
Ppp2ca
ORF Size (aa)
930
RefSeq ID
NM_017039
RefSeq Synonyms
NM_017039, BC072531, BC070914,
Species
rat
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.