human IDH3B Over-expression AAV

Name 
AAV-h-IDH3B
Cat No 
AAV-211774
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human IDH3B.

AAV-211774
AAV-h-IDH3B

Ready-to-use AAV expressing Human IDH3B (isocitrate dehydrogenase 3 (NAD(+)) beta). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
isocitrate dehydrogenase NAD subunit beta, mitochondrial; isocitrate dehydrogenase 3 (NAD+) beta; isocitric dehydrogenase subunit beta; NAD(+)-specific ICDH subunit beta; RP46
Description (eCommerce)
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit.Isocitric dehydrogenase subunit beta (IDH3B) is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase [taken from NCBI Entrez Gene (Gene ID: 3420)].
Description (Vector)
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Gene ID
3420
Gene Name (eCommerce)
isocitrate dehydrogenase 3 (NAD(+)) beta
Gene Name (Vector)
isocitrate dehydrogenase 3 (NAD(+)) beta
Gene Symbol
IDH3B
HGNC ID
HGNC:5385
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1158
ORF Size (bp)
1158 bp
Protein Name (eCommerce)
Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
RefSeq ID
NM_174856
RefSeq Synonyms
NM_174856, NM_174855, NM_006899, NM_001330763, NM_001258384, BC001960,
Research Areas
Metabolism/Metabolic Process,Mitochondrion
Research Areas (Faceted)
cell_biology,metabolism
Species
human
Target Sentence
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit.Isocitric dehydrogenase subunit beta (IDH3B) is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase [taken from NCBI Entrez Gene (Gene ID: 3420)].
UniGene ID
Hs.436405
UniProt ID (eCommerce)
O43837

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.