rat AS3MT shRNA silencing AAV

Name
AAV-r-AS3MT-shRNA

Cat No
shAAV-290096

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Rat As3mt.

shAAV-290096

AAV-r-AS3MT-shRNA

Ready-to-use AAV expressing shRNA for silencing of Rat As3mt (arsenite methyltransferase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

Cyt19

Description (Vector)

Catalyzes the transfer of a methyl group from AdoMet to trivalent arsenicals producing methylated and dimethylated arsenicals. It methylates arsenite to form methylarsonate, Me-AsO(3)H(2), which is reduced by methylarsonate reductase to methylarsonite, Me-As(OH)2. Methylarsonite is also a substrate and it is converted into the much less toxic compound dimethylarsinate (cacodylate), Me(2)As(O)-OH (By similarity).

Gene ID

140925

Gene Name (Vector)

arsenite methyltransferase

Gene Symbol

As3mt

ORF Size (aa)

699

ORF Size (bp)

699 bp

RefSeq ID

NM_080890

RefSeq Synonyms

NM_080890, BC061533,

Species

rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.