mouse SS18L1 shRNA silencing AAV

Name
AAV-m-SS18L1-shRNA

Cat No
shAAV-273170

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Mouse Ss18l1.

shAAV-273170

AAV-m-SS18L1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Ss18l1 (SS18, nBAF chromatin remodeling complex subunit like 1). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

A230053O16Rik; CREST

Description (Vector)

Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity).

Gene ID

269397

Gene Name (Vector)

SS18, nBAF chromatin remodeling complex subunit like 1

Gene Symbol

Ss18l1

ORF Size (aa)

1209

RefSeq ID

NM_178750

RefSeq Synonyms

NM_178750, BC054730, BC053087,

Species

mouse

UniGene ID

Mm.63068

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.