human HCRT Over-expression AAV

Name 
AAV-h-HCRT
Cat No 
AAV-210877
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human HCRT.

AAV-210877
AAV-h-HCRT

Ready-to-use AAV expressing Human HCRT (hypocretin neuropeptide precursor). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
NRCLP1; OX; PPOX
Description (Vector)
HCRT encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis.
Gene ID
3060
Gene Name (Vector)
hypocretin neuropeptide precursor
Gene Symbol
HCRT
HGNC ID
HGNC:4847
ORF Size (aa)
393
RefSeq ID
NM_001524
RefSeq Synonyms
NM_001524, BC111915,
Species
human
UniGene ID
Hs.158348

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.