mouse RPE65 shRNA silencing AAV

Name
AAV-m-RPE65-shRNA

Cat No
shAAV-270828

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Mouse Rpe65.

shAAV-270828

AAV-m-RPE65-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Rpe65 (retinal pigment epithelium 65). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

65kDa; A930029L06Rik; LCA2; Mord1; rd12; RP20

Description (Vector)

Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.

Gene ID

19892

Gene Name (Vector)

retinal pigment epithelium 65

Gene Symbol

Rpe65

ORF Size (aa)

1602

ORF Size (bp)

1602 bp

RefSeq ID

NM_029987

RefSeq Synonyms

NM_029987, BC130028,

Species

mouse

UniGene ID

Mm.131708

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.