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mouse PPME1 shRNA silencing AAV

Name 
AAV-m-PPME1-shRNA
Cat No 
shAAV-269179
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Ppme1.

shAAV-269179
AAV-m-PPME1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Ppme1 (protein phosphatase methylesterase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
1110069N17Rik; 2700017M01Rik; PME-1; Pme1
Description (Vector)
Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme.
Gene ID
72590
Gene Name (Vector)
protein phosphatase methylesterase 1
Gene Symbol
Ppme1
ORF Size (aa)
1161
ORF Size (bp)
1161 bp
RefSeq ID
NM_028292
RefSeq Synonyms
NM_028292, BC029064, BC014867,
Species
mouse
UniGene ID
Mm.477484

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.