mouse PCGF2 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Pcgf2.
shAAV-268210
AAV-m-PCGF2-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Pcgf2 (polycomb group ring finger 2). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
mel-18; Mel18; Rnf110; Zfp144
Description (Vector)
Transcriptional repressor. Binds specifically to the DNA sequence 5'-GACTNGACT-3'. Has tumor suppressor activity. May play a role in control of cell proliferation and/or neural cell development. Regulates proliferation of early T progenitor cells by maintaining expression of HES1. Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Is not functionally redundant with Bmi1; unlike Bmi1 does not stimulate the E3 ubiquitin-protein ligase activity in a reconstituted PRC1-like complex (By similarity).
Gene ID
22658
Gene Name (Vector)
polycomb group ring finger 2
Gene Symbol
Pcgf2
ORF Size (aa)
1029
RefSeq ID
NM_009545
RefSeq Synonyms
NM_009545, NM_001163308, NM_001163307, BC016419,
Species
mouse
UniGene ID
Mm.2418
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.