mouse NR5A1 shRNA silencing AAV

Name
AAV-m-NR5A1-shRNA

Cat No
shAAV-266060

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Mouse Nr5a1.

shAAV-266060

AAV-m-NR5A1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Nr5a1 (nuclear receptor subfamily 5, group A, member 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

Ad4BP; ELP; ELP-3; Ftz-F1; Ftzf1; SF-1; SF1; STF-1

Description (Vector)

Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. Binds phosphatidylcholine (By similarity). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.

Gene ID

26423

Gene Name (Vector)

nuclear receptor subfamily 5, group A, member 1

Gene Symbol

Nr5a1

ORF Size (aa)

1389

ORF Size (bp)

1389 bp

RefSeq ID

NM_139051

RefSeq Synonyms

NM_139051, NM_001316687, BC110477, BC110476,

Species

mouse

UniGene ID

Mm.31387

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.