mouse NIT1 shRNA silencing AAV

Name 
AAV-m-NIT1-shRNA
Cat No 
shAAV-265791
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Nit1.

shAAV-265791
AAV-m-NIT1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Nit1 (nitrilase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
60
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
AI255805; ESTM30; W57327
Description (Vector)
Plays a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2 (By similarity).
Gene ID
27045
Gene Name (Vector)
nitrilase 1
Gene Symbol
Nit1
ORF Size (aa)
972
ORF Size (bp)
972 bp
RefSeq ID
NM_012049
RefSeq Synonyms
NM_012049, NM_001242580, BC021634,
Species
mouse
UniGene ID
Mm.12915

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.