mouse EZH1 shRNA silencing AAV

Name
AAV-m-EZH1-shRNA

Cat No
shAAV-258821

Availability
Immediate

This is an AAV expressing shRNA for silencing of Mouse Ezh1.

shAAV-258821

AAV-m-EZH1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Ezh1 (enhancer of zeste 1 polycomb repressive complex 2 subunit). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

ENX-2

Description (Vector)

Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.

Gene ID

14055

Gene Name (Vector)

enhancer of zeste 1 polycomb repressive complex 2 subunit

Gene Symbol

Ezh1

ORF Size (aa)

2253

ORF Size (bp)

2253 bp

RefSeq ID

NM_007970

RefSeq Synonyms

NM_007970, BC007135,

Species

mouse

UniGene ID

Mm.5027

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.