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mouse E4F1 shRNA silencing AAV

Name 
AAV-m-E4F1-shRNA
Cat No 
shAAV-257683
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse E4f1.

shAAV-257683
AAV-m-E4F1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse E4f1 (E4F transcription factor 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
50
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
p120E4F; phi-AP3
Description (Vector)
E4F1 may function as a transcriptional repressor. May also function as a ubiquitin ligase mediating ubiquitination of chromatin-associated TP53. Functions in cell survival and proliferation through control of the cell cycle. Functions in the p53 and pRB tumor suppressor pathways and regulates the cyclin CCNA2 transcription|Function: Identified as a cellular target of the adenoviral oncoprotein E1A, it is required for both transcriptional activation and repression of viral genes.
Gene ID
13560
Gene Name (Vector)
E4F transcription factor 1
Gene Symbol
E4f1
ORF Size (aa)
1605
ORF Size (bp)
1605 bp
RefSeq ID
NM_007893
RefSeq Synonyms
NM_007893, NM_001301784, BC071228, BC046459,
Species
mouse
UniGene ID
Mm.163132

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.