mouse CHFR shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Chfr.
shAAV-255387
AAV-m-CHFR-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Chfr (checkpoint with forkhead and ring finger domains). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
5730484M20Rik; C230082M18; RNF116
Description (Vector)
E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.
Gene ID
231600
Gene Name (Vector)
checkpoint with forkhead and ring finger domains
Gene Symbol
Chfr
ORF Size (aa)
1995
ORF Size (bp)
1995 bp
RefSeq ID
NM_172717
RefSeq Synonyms
NM_172717, NM_001289580, NM_001289579, NM_001289578, NM_001289577, BC049792,
Species
mouse
UniGene ID
Mm.30264
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.