mouse BTRC shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Btrc.
shAAV-254168
AAV-m-BTRC-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Btrc (beta-transducin repeat containing protein). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
b-TrCP; beta-TrCP; Beta-Trcp1; E3RS-IkappaB; E3RSIkappaB; Fbw1a; FWD1; HOS; Slimb
Description (Vector)
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22'. SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1/nuclear factor NF-kappa-B p105 subunit, ATF4, SMAD3, SMAD4, CDC25A, DLG1, FBXO5 and probably NFKB2. SCF(BTRC) mediates the ubiquitination of phosphorylated SNAI1. May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase. Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF. Required for proteolytic processing of GLI3.
Gene ID
12234
Gene Name (Vector)
beta-transducin repeat containing protein
Gene Symbol
Btrc
ORF Size (aa)
1710
ORF Size (bp)
1710 bp
RefSeq ID
NM_009771
RefSeq Synonyms
NM_009771, NM_001286466, NM_001286465, NM_001037758, BC003989,
Species
mouse
UniGene ID
Mm.446448
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.