mouse ACCN2 shRNA silencing AAV

Name
AAV-m-ACCN2-shRNA

Cat No
shAAV-251897

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Mouse Asic1.

shAAV-251897

AAV-m-ACCN2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Asic1 (acid-sensing (proton-gated) ion channel 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

Accn2; AI843610; ASIC; ASIC1a; B530003N02Rik; BNaC2

Description (Vector)

Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear (By similarity).

Gene ID

11419

Gene Name (Vector)

acid-sensing (proton-gated) ion channel 1

Gene Symbol

Asic1

ORF Size (aa)

1581

ORF Size (bp)

1581 bp

RefSeq ID

NM_009597

RefSeq Synonyms

NM_009597, NM_001289791, BC067025,

Species

mouse

UniGene ID

Mm.440107

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.