mouse RPTOR shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Rptor.
shAAV-251238
AAV-m-RPTOR-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Rptor (regulatory associated protein of MTOR, complex 1). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
4932417H02Rik; mKIAA1303; Rap; Raptor
Description (Vector)
Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.
Gene ID
74370
Gene Name (Vector)
regulatory associated protein of MTOR, complex 1
Gene Symbol
Rptor
ORF Size (aa)
4008
ORF Size (bp)
4008 bp
RefSeq ID
NM_028898
RefSeq Synonyms
NM_028898, NM_001306081, BC167200,
Species
mouse
UniGene ID
Mm.209933
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.