human ALOXE3 shRNA silencing AAV

Name
AAV-h-ALOXE3-shRNA

Cat No
shAAV-230067

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human ALOXE3.

shAAV-230067

AAV-h-ALOXE3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ALOXE3 (arachidonate lipoxygenase 3). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

ARCI3; E-LOX; eLOX-3; eLOX3

Description (Vector)

ALOXE3 is a member of the lipoxygenase family, which are catabolized by arachidonic acid-derived compounds. The encoded enzyme is a hydroperoxide isomerase that synthesizes a unique type of epoxy alcohol (8R-hydroxy-11R,12R-epoxyeicosa-5Z,9E,14Z-trienoic acid) from 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE). This epoxy alcohol can activate the the nuclear receptor peroxisome proliferator-activated receptor alpha (PPARalpha), which is implicated in epidermal differentiation. Loss of function of the enzyme encoded by this gene results in ichthyosis, implicating the function of this gene in the differentiation of human skin. ALOXE3 is part of a cluster of lipoxygenase genes on 17p13.1. Mutations in this gene result in nonbullous congenital ichthyosiform erythroderma (NCIE). Multiple transcript variants encoding different isoforms have been found for this gene.

Gene ID

59344

Gene Name (Vector)

arachidonate lipoxygenase 3

Gene Symbol

ALOXE3

HGNC ID

HGNC:13743

ORF Size (aa)

2136

ORF Size (bp)

2136 bp

RefSeq ID

NM_021628

RefSeq Synonyms

NM_021628, NM_001165960, BC104724, BC103508, BC101939, BC101938,

Species

human

UniGene ID

Hs.694602

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.