human INS shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human INS.
shAAV-229744
AAV-h-INS-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human INS (insulin). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
IDDM; IDDM1; IDDM2; ILPR; IRDN; MODY10
Description (Vector)
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants.
Gene ID
3630
Gene Name (Vector)
insulin
Gene Symbol
INS
HGNC ID
HGNC:6081
ORF Size (aa)
333
ORF Size (bp)
333 bp
RefSeq ID
NM_000207
RefSeq Synonyms
NM_001291897, NM_001185098, NM_001185097, NM_000207, BC005255,
Species
human
UniGene ID
Hs.
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.