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human TFE3 shRNA silencing AAV

Name 
AAV-h-TFE3-shRNA
Cat No 
shAAV-225357
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human TFE3.

shAAV-225357
AAV-h-TFE3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human TFE3 (transcription factor binding to IGHM enhancer 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
bHLHe33; class E basic helix-loop-helix protein 33; RCCP2; RCCX1; TFEA; transcription factor E family, member A; transcription factor E3; transcription factor for IgH enhancer; transcription factor for immunoglobulin heavy-chain enhancer 3
Description (eCommerce)
Transcription factor E3 (TFE3) is a member of the microphthalmia transcription factor/transcription factor E (MITF-TFE) family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors. This family includes four members: MITF, TFE3, TFEB and TFEC. TFE3 activates transcription through binding to the muE3 motif of the immunoglobulin heavy-chain enhancer [taken from NCBI Entrez Gene (GeneID: 7030)].
Description (Vector)
The microphthalmia transcription factor/transcription factor E (MITF-TFE) family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors includes four family members: MITF, TFE3, TFEB and TFEC. The TEF3 protein encoded by this gene activates transcription through binding to the muE3 motif of the immunoglobulin heavy-chain enhancer. The TFEC protein forms heterodimers with the TEF3 protein and inhibits TFE3-dependent transcription activation. The TEF3 protein interacts with transcription regulators such as E2F3, SMAD3, and LEF-1, and is involved in TGF-beta-induced transcription, playing important roles in cell growth, proliferation, and osteoclast and macrophage differentiation. The TFE3 protein also activates hepatic IRS-2 gene, and induces hexokinase II (HK2) and insulin-induced gene 1 (INSIG1); it participates in insulin signaling and could be a therapeutic target for diabetes. This gene is also involved in chromosomal translocations, resulting in different fusion gene products in papillary renal cell carcinomas and alveolar soft part sarcomas, such as PRCC-TFE3, RCC17-TFE3, PSF-TFE3, NonO (p54nrb)-TFE3 and ASPL-TFE3.
Gene ID
7030
Gene Name (eCommerce)
transcription factor binding to IGHM enhancer 3
Gene Name (Vector)
transcription factor binding to IGHM enhancer 3
Gene Symbol
TFE3
HGNC ID
HGNC:11752
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1728
ORF Size (bp)
1728 bp
Protein Name (eCommerce)
Transcription factor E3
RefSeq ID
NM_006521
RefSeq Synonyms
NM_006521, NM_001282142, BC026027,
Research Areas
Cancer,Immunology,Signal Transduction,TGF-beta Signaling,Transcription Factor/Regulator,Transcriptional Misregulation in Cancer
Research Areas (Faceted)
cancer,cell_biology,immunology,signal_transduction,transcription_translation
Species
human
Target Sentence
Transcription factor E3 (TFE3) is a member of the microphthalmia transcription factor/transcription factor E (MITF-TFE) family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors. This family includes four members: MITF, TFE3, TFEB and TFEC. TFE3 activates transcription through binding to the muE3 motif of the immunoglobulin heavy-chain enhancer [taken from NCBI Entrez Gene (GeneID: 7030)].
UniGene ID
Hs.274184
UniProt ID (eCommerce)
P19532

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.