human TFE3 shRNA silencing Adenovirus

Name
Ad-h-TFE3-shRNA

Cat No
shADV-225357

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human TFE3.

shADV-225357

Ad-h-TFE3-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human TFE3 (transcription factor binding to IGHM enhancer 3). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

bHLHe33; class E basic helix-loop-helix protein 33; RCCP2; RCCX1; TFEA; transcription factor E family, member A; transcription factor E3; transcription factor for IgH enhancer; transcription factor for immunoglobulin heavy-chain enhancer 3

Description (eCommerce)

Transcription factor E3 (TFE3) is a member of the microphthalmia transcription factor/transcription factor E (MITF-TFE) family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors. This family includes four members: MITF, TFE3, TFEB and TFEC. TFE3 activates transcription through binding to the muE3 motif of the immunoglobulin heavy-chain enhancer [taken from NCBI Entrez Gene (GeneID: 7030)].

Description (Vector)

The microphthalmia transcription factor/transcription factor E (MITF-TFE) family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors includes four family members: MITF, TFE3, TFEB and TFEC. The TEF3 protein encoded by this gene activates transcription through binding to the muE3 motif of the immunoglobulin heavy-chain enhancer. The TFEC protein forms heterodimers with the TEF3 protein and inhibits TFE3-dependent transcription activation. The TEF3 protein interacts with transcription regulators such as E2F3, SMAD3, and LEF-1, and is involved in TGF-beta-induced transcription, playing important roles in cell growth, proliferation, and osteoclast and macrophage differentiation. The TFE3 protein also activates hepatic IRS-2 gene, and induces hexokinase II (HK2) and insulin-induced gene 1 (INSIG1); it participates in insulin signaling and could be a therapeutic target for diabetes. This gene is also involved in chromosomal translocations, resulting in different fusion gene products in papillary renal cell carcinomas and alveolar soft part sarcomas, such as PRCC-TFE3, RCC17-TFE3, PSF-TFE3, NonO (p54nrb)-TFE3 and ASPL-TFE3.

Gene ID

7030

Gene Name (eCommerce)

transcription factor binding to IGHM enhancer 3

Gene Name (Vector)

transcription factor binding to IGHM enhancer 3

Gene Symbol

TFE3

HGNC ID

HGNC:11752

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

1728

ORF Size (bp)

1728 bp

Protein Name (eCommerce)

Transcription factor E3

RefSeq ID

NM_006521

RefSeq Synonyms

NM_006521, NM_001282142, BC026027,

Research Areas

Cancer,Immunology,Signal Transduction,TGF-beta Signaling,Transcription Factor/Regulator,Transcriptional Misregulation in Cancer

Research Areas (Faceted)

cancer,cell_biology,immunology,signal_transduction,transcription_translation

Species

human

Target Sentence

UniGene ID

Hs.274184

UniProt ID (eCommerce)

P19532

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.