human SHARPIN shRNA silencing AAV

Name
AAV-h-SHARPIN-shRNA

Cat No
shAAV-222924

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human SHARPIN.

shAAV-222924

AAV-h-SHARPIN-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human SHARPIN (SHANK associated RH domain interactor). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

90

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

hSIPL1; shank-associated RH domain-interacting protein; shank-interacting protein-like 1; sharpin; SIPL1

Description (eCommerce)

Sharpin (Shank-associated RH domain-interacting protein) is a component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9H0F6].

Gene ID

81858

Gene Name (eCommerce)

SHANK associated RH domain interactor

Gene Name (Vector)

SHANK associated RH domain interactor

Gene Symbol

SHARPIN

HGNC ID

HGNC:25321

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

1164

ORF Size (bp)

1164 bp

Protein Name (eCommerce)

Sharpin

RefSeq ID

NM_030974

RefSeq Synonyms

NM_030974, BC034028, BC025244,

Research Areas

Apoptosis,Brain Development,Cancer,CD40 Signaling,Immunology,Neurobiology,Neurodevelopment,NF-kB Signaling,Signal Transduction,Synapse,Ubiquitination,Zinc-finger

Research Areas (Faceted)

cancer,cell_biology,developmental_biology,immunology,neurobiology,signal_transduction

Species

human

Target Sentence

Sharpin (Shank-associated RH domain-interacting protein) is a component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9H0F6].

UniGene ID

Hs.529755

UniProt ID (eCommerce)

Q9H0F6

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.