human SEMA4A shRNA silencing AAV

Name
AAV-h-SEMA4A-shRNA

Cat No
shAAV-222549

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human SEMA4A.

shAAV-222549

AAV-h-SEMA4A-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human SEMA4A (semaphorin 4A). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

CORD10; RP35; SEMAB; SEMB

Description (Vector)

SEMA4A encodes a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in numerous functions, including axon guidance, morphogenesis, carcinogenesis, and immunomodulation. The encoded protein is a single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. It is an activator of T-cell-mediated immunity and suppresses vascular endothelial growth factor (VEGF)-mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. Mutations in this gene are associated with retinal degenerative diseases including retinitis pigmentosa type 35 (RP35) and cone-rod dystrophy type 10 (CORD10). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

Gene ID

64218

Gene Name (Vector)

semaphorin 4A

Gene Symbol

SEMA4A

HGNC ID

HGNC:10729

ORF Size (aa)

2286

ORF Size (bp)

2286 bp

RefSeq ID

NM_022367

RefSeq Synonyms

NM_022367, NM_001193302, NM_001193301, NM_001193300, BC020974,

Species

human

UniGene ID

Hs.408846

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.